Basic and clinical science posters: Basic science of diabetes complications

نویسندگان

چکیده

Aims: Glucose evoked changes in profibrotic transforming growth factor-beta1 (TGFs1), increase connexin hemichannel mediated ATP release kidney proximal tubule cells. With linked to inflammation, we evaluated the efficacy of blocker Peptide 5 negating induced inflammatory cytokine secretion cells. Methods: Primary human tubular epithelial cells (hPTECs), and immortalised (HK2) were treated with TGFs1 (10ng/ml) ± Peptide5 (25µM) for 48hrs. Carboxyfluorescein (200µM) dye uptake biosensing determined activity respectively. Proteome arrays secretion. Results: In hPTECs, carboxyfluorescein decreased from 347.4±11.9%, (P<0.001, N=3) 158.6±7.9% when co-incubated 5. Parallel this, increased 0.16±0.1µM 2.5±0.14µM TGF1 as compared control. significantly blunted this response, 0.62±0.17µM N=3). A proteome array impact on 105 candidate chemokines cytokines primary As an example, restored adiponectin 163±8.0% N=4) 136±6.1% (P<0.05, N=4), granulocyte-macrophage colony-stimulating factor 183±8.0% 111.3±5.9% interleukin 1-beta 189±14.9 109±9.6% TGF-s1 control. Conclusion: release. Hemichannel blocker; blocks downstream chemokine secretion, suggesting that may be future therapeutic potential targeting inflammation diabetic kidney.

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ژورنال

عنوان ژورنال: Diabetic Medicine

سال: 2021

ISSN: ['1464-5491', '1096-9136', '0742-3071']

DOI: https://doi.org/10.1111/dme.2_14556